The function to output AC voltage is added to Genome Editor, which enhanced zygote genome editing researches.
Two mode: AC + DC pulses for oocyte activation and tetraploid formation; and DC pulses for zygote genome editing.
Easy-to-use operation to program the parameter by touch panel
All the data of executed pulse output parameters can be exported through USB memory.
All the electrodes for CUY electroporator series and LF electrofusion series are connectable.
High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote
Electrode for zygote electroporation
LF501PT1-10 is applied for zygote genome editing, newly developed for zygoe electroporation.
Comparison between electroporation and microinjection
Electroporation | Microinjection | |
|---|---|---|
Pre-operation | Not necessary | Preparation of injection and hold pippettes, etc. |
Required time | ~5 minutes | > 2 hours |
Viability (after E15) | 40-80% | 10-50% |
Efficiency | Same as microinjection | Depends on the sequence |
Cost for devices | About $12,000 | > $50,000 |
Required skills | Not necessary | Maniipularion of single zygotes |
Required amount of Cas9 mRNA | 500 - 2,000 ng | 50 - 500 ng |
More zygotes can be treated at one time by electroporation than microinjection.
Viability of treated zygotes are much higher in electroporation than in microinjection.
No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.
Electroporation is , therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.
